What corrections were made to the NCBI data?
Probe queries in SpliceMiner require an exact mapping of transcripts to chromosomal coordinates. In a small percentage of NCBI records, there is an internal inconsistency between the genomic and transcript coordinates. This most often occurs when there are insertions or deletions in the transcript relative to the genome sequence. In cases where the length of an exon in transcript coordinates was not equal to the length of the exon in chromosomal coordinates, a refinement was performed by re-aligning the exon to the genomic sequence using BLAT. Exon fragment records were then created to indicate the exact alignment of each section of the transcript to the genome. The corrected exon records enable SpliceMiner to accurately determine the chromosomal position of probe sequences. Corrected exon records are identified by a 1 in the Coord-Correction column of batch results.
