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What conditions will help reduce NSB to MG-CM particles?

April 26, 2017Conditions help nsb particles reduce

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What conditions will help reduce NSB to MG-CM particles?

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In order to minimize adsorptive binding to Sera-Mag the suspending buffer should be at elevated pH (for example, greater than or equal to 8)and combine high salt (NaCl >= 150 mM) and nonionic surfactant (Tween 20 0.1 %). The composition of the SA MP suspension buffer is 50 mM Tris, pH 8.0; NaCl 150 mM; Tween 20 0.1%. This combination gave quite low adsorptive binding of serum proteins to Sera-Mag particles.