What concentrations of compounds can be used in HTS assays?
We typically suggest screening at about 10 uM. Lower concentrations may be used in cell-based assays to reduce false positives and to bias detection toward more potent compounds. For “difficult” targets it may be useful to screen with higher concentrations (20-100 uM) but the number of false positive results is usually quite a bit higher resulting in more work and expense in subsequent studies. Dose response studies are typically done up to 100 uM (perhaps 300 uM).
