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What concentration of G418 should I use to construct a stable cell line with pNEBR-R1?

April 26, 2017CELL concentration construct g418 line pnebr-r1 STABLE

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What concentration of G418 should I use to construct a stable cell line with pNEBR-R1?

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Each mammalian cell line has different sensitivities to different antibiotics. The sensitivity can be determined by performing a standard kill curve. The least amount of antibiotic that results in 100% lethality over the course of a few days should be used for subsequent experiments.