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What cloning strategy is recommended for TripleMaster amplified DNA?

April 26, 2017amplified cloning dna recommended strategy triplemaster

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What cloning strategy is recommended for TripleMaster amplified DNA?

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With TripleMaster amplified DNA, there is a mixture of amplified DNA with blunt-ends and A-overhangs. Both blunt- and TA-cloning are possible. With TA-cloning we possibly recommend Taq re-incubation to create additional A-overhangs.