What chemicals do bind to the cartridge in the Bind-ETTM?
A21: The Bind-ETTM was designed to purify any inorganic solution (e.g. salt buffers used for electrophoresis, e.g. TAE, TBE) from certain chemical compounds such as DNA-staining dyes. Do not use the Bind-ET to purify solutions containing more than 10% organic solvents! Chemical compounds bind to resins inside the cartridge of a Bind-ET by an ion exchange reaction. This reaction is not limited to the binding of Ethidium Bromide (EtBr) but also eliminates other fluorescent dyes (such as SYBR Gold / Green) from the flow-through. However, it is crucial that the flow rate does not exceed 3 L per hour to guarantee complete removal of the compound from the buffer.
