What cell lines can I use with the SureSilencing™ shRNA plasmids?
The SureSilencing™ shRNA plasmids are designed for stable, transformed human, mouse, or rat cell lines. We currently cannot support RNA interference for any other species. The plasmids are not suitable for in vivo work. They are also not designed, nor meant for use with, primary cell lines, macrophages, or other cell lines that tend to be very difficult to transfect. For these other applications, we instead recommend finding a viral-based delivery system.
Related Questions
- How can I use the SureSilencing™ shRNA plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin?
- Does the same U1 promoter in the SureSilencing™ shRNA plasmids express shRNA in human, mouse, and rat cell lines?
- What cell line does SABiosciences use for quality control testing of the SureSilencing™ shRNA plasmids?
