What can cause grid to grid variability on the array?
Ensure that there are no air bubbles trapped under the coverslip. Also ensure handling of the dendrimers is as specified in the manual. if the dendrimers are aggregate it can lead to signal variability. One other common contributor to variability is with the coverslips used. We see less variability using the 25×60 slips. If too narrow or short the array does not get uniform coverage . The boundary of the lifter slip needs to be as far from the array as possible. Larger wash tubes are preferred. Our wash tubs hold around 500ml of wash buffer. Overall, variability is usually due to uneven hyb across the surface of the array. This is much more pronounced when static hybs are done compared to when mixing devices are used.
