What can be seen using an electron microscope in relation to cell structure?
I don’t think you can’t see rough ER through a light microscope, unless you would use a specific fluorescent stain, but you could see the nucleus and barely but just mitochondria, if present chloroplasts. Think what other organelles there are. Look in your textbook for a list if you need help. In general the resolution limit of a good light microscope is 200 nanometer, so you won’t see anything smaller than that. That might help you to figure out what you can see and what you can’t. For membranous organelles like ER or Golgi apparatus, remember that you need to see the membranes in order to see the extent of the organelle unless the optical density of the inside is noticably different from the outside. How big is a ribosome? How thick is a cell membrane? Generally a lot will depend on what stain you will use. If you use specific antibodies which are coupled with a fluorescent dye you can see the location of objects which are otherwise too small to resolve with a light microscope. If yo
