What buffers are compatible with the eFluor® Nanocrystal conjugated molecules?
We have determined that staining of cells in the recommended eFluor® NC Flow Cytometry Staining Buffer (cat. 00-3222) provides the most consistent results with the eFluor® Nanocrystals. While we do see staining in PBS based-buffers, we observe slightly brighter signal intensity when using eFluor® NC Flow Cytometry Staining Buffer with the eFluor® Nanocrystals.
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