What buffer should I use to prepare my cell lysate samples?
Please check the recommended buffer in the Western blotting protocol included with each product’s data sheet. The lysis buffers are also available as products (see below), or the specific buffer components are listed on the CST website if you would like to prepare them yourself. Please note: It is absolutely critical to use CHAPS Buffer (10X) #9852 in preparing cell extracts with the antibodies for caspases and caspase substrates.
