What buffer should I resuspend my oligo in?
We recommend preparing stock and working solutions using TE (10mM Tris, 1mM EDTA pH 7.5-8.0) or sterile, DNase-free water. DEPC treated water is not recommended, because it has been known to chemically modify adenine residues and inhibit downstream applications such as enzymatic reactions. Please note that fluorescent probes are more sensitive than primers and require more care in handling. Rhodamine based dyes, such as TAMRA and ROX are relatively pH insensitive. But for our Quasar 570, Quasar 670 and other dyes such as Cy™3 and Cy5, are physically unstable in acidic conditions and therefore should be stored and used in buffers above pH 7 to prevent degradation. Oligonucleotides resuspended in water may not dissolve fully in sterile distilled water. Oligos resuspended in water tend to have a pH around 5, and this is often too low for them to completely dissolve. If you resuspend in water, you may need to add NaOH to raise the pH between 7 and 8. If your experiment cannot tolerate EDTA
