Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

What blotting membranes are compatibles with SNAP i.d. System?

April 26, 2017blotting compatibles Membranes snap system

0

Posted

What blotting membranes are compatibles with SNAP i.d. System?

1 Answer

0

Posted

• All standard (nitrocellulose and PVDF) membranes are compatible. For optimal performance we recommend Immobilon-P membrane for chemiluminescent detection or Immobilon- FL membrane for fluorescent detection. Q. Can the SNAP i.d. System be used right after blot transfer? • Yes, as long as the blot is still wet. Q. Is any danger of drying the blot if the vacuum is applied for too long? • No, even if you keep the vacuum running for several minutes the blot will not dry since some liquid always remains within the blot holder. Blocking Q. Can I use non-fat dry milk (NFDM) for blocking in the SNAP i.d. System? • Yes, NFDM is excellent blocking reagent for the SNAP i.d. Detection System. The optimal concentration of NFDM in SNAP i.d. is 0.5%. Q. I only have low-fat dry milk available. Can I use that with the SNAP i.d. System? • Yes, follow the same recommendations that are made for using non-fat dry milk. Q. Can I use NFDM at concentration lower than 0.5%? • Yes, we have found adequate block