What assay does FBR use, and how does FBR assure the quality of its cotinine assay?
The cotinine assay is a double antibody competitive inhibition radioimmunoassay, developed here at FBR. Monitoring includes the use of control samples placed at the beginning, middle, and end of each assay. Control levels are compared within and between assays in order to detect any drift or assay errors, should they occur. If control values are outside of acceptable levels, the results from that run are discarded, the error identified and corrected, and the samples re-assayed. Additionally, urine specimens are serially diluted in order to assure that an accurate value is obtained from the optimum part of the assay’s curve.
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