What are your recommendations with regards to endotoxin-free DNA for transfections?
Some researchers prefer endotoxin-free DNA for their transfection experiments if they are working with cells that are particularly sensitive to endotoxins. To prepare endotoxin-free DNA, Invitrogen provides a number of nucleic acid purification kits called “PureLink⢠HiPure Purification Kits” in Mini, Midi, Maxi, Mega and Giga sizes. For more information, please click here.