What are the techniques of Purification of Proteins?
In order to identify the working mechanism of a particular protein, it is essential to know about the sequence of its amino acids and their nature. Once the purification of protein is accomplished, it is simple to determine the sequence of amino acids. Since after the application of appropriate purifying techniques, a sample will be yielded which contains only one protein which is the protein of interest. The proteins need to be released out the cell and can be separated into different densities using the method of differential centrifugation. Proteins can be distinguished from one another on the basis of their size, solubility, charges contained and the binding capacity. The following are the popular methods of proteins purification. I got these information from examville.com when I was studying for Bsc final year.
