What are the technical details of the Walk Through the Y (WTY) Test?
The test includes Sanger sequencing of five plates of ninety-six Y-chromosome PCR segments each. We sequence in both the forward and the reverse directions. Thus, the total number of unique bases sequenced on the Y-chromosome will usually exceed 180 kB. The DNA segments chosen can be considered as randomly chosen regions on the Y-chromosome which are unlikely to recombine. There will be no reruns (unless the sample failed completely or produced results for less than half of the expected sequence). All known derived SNPs and all differences from the HUGO reference sequence will be scored. Should new SNPs be found, Family Tree DNA will submit new public SNPs to NCBI (for an RS number), but we cannot guarantee that they will be accepted. If a customer publishes a new marker, all involved laboratory personnel at our Houston, Texas, Genomics Research Center (GRC) must be added as co-authors.
