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What are the storage size requirements for the sequence data output from a single run?

April 26, 2017Data output requirements run sequence size storage

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What are the storage size requirements for the sequence data output from a single run?

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To process the data from a single run, the system requires 500 GB to 1 TB (depending on factors such as cycles and cluster density). Images are acquired and stored on the instrument workstation and must then be transferred to an external computer to be analyzed by the Genome Analyzer Pipeline software, which handles image processing, base calling, and sequence alignment. The software runs on all common Unix/Linux variants. A high-end Linux box should be adequate as the analysis computer. However, Illumina software is compatible with Sun Grid Engine and LSF, if you wish to install it on a cluster. The instrument generates ~1 Tb of data during a full 2–3 day run. About 70% of this is TIFF image data that can be sent to tape after a run is finished and you are satisfied a reanalysis is not required.