What are the steps in performing the AcycloPrime assay?
• Isolation & amplification of genomic DNA (PCR or other) • Degradation of primers and free nucleotides using the PCR Clean-up Reagents provided in the AcycloPrime kit • 3′-End labeling of the SNP primer with dye-AcycloTerminator(s) using AcycloPol • Detection of incorporated dye-AcycloTerminators by fluorescence polarization (FP) using the Victor2 or another instrument.
