What are the specifications of polyclonal antibodies and how is quality assured?
The antibodies pass QC specs if they are able to recognize the antigen used for immunization at a dilution of the serum not lower than 1:1,000. As negative control, we collect the pre-immune serum, which is used for internal QC. The pre-immune serum will be delivered to you together with the antibodies. Serum collected from the animals is tested by ELISA. According to our procedures, the antigen used for immunization is coated on a microtiter plate and allowed to react with the specific serum. The reaction is followed by labelled secondary antibodies, which are able to recognize the specific immunoglobulins bound to the antigen. The intensity of the resulting label signals provides a quantitative measure of the antibodies. The use of pre-immune sera allows to establish the specificity of the antibodies. In case of antibodies generated against synthetic peptides, the free un-conjugated peptide is coated on the microtiter plate. This way, we do not measure the immunoglobulins raised agai
