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What are the specific requirements for SSR development?

April 26, 2017Development requirements ssr

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What are the specific requirements for SSR development?

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DNA adaptors (2) Motif oligos (8-12) SP6 and T7 plasmid-primers Rsa I restriction enzyme Ligase enzyme Nylon membranes – positively charged PCR kit (plus dNTPs) Pre and post hybridisation buffers SSC SDS PCR cleanup kit Cloning kit (with competent Ecoli) Plating/screening media Miniprep kit Sequencing (20) Primer design software Fluorescent primer sets