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WHAT ARE THE SIZE LIMITS FOR THE AMPLIFIED TARGET DNA SEQUENCE TO BE USED IN HYB & SEQ?

April 26, 2017amplified dna hyb limits SEQ sequence size target Used

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WHAT ARE THE SIZE LIMITS FOR THE AMPLIFIED TARGET DNA SEQUENCE TO BE USED IN HYB & SEQ?

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We have successfully used amplified DNA fragments from 100 to 1000 bp. However, usage of large DNA fragments seems to lower the signals, to increase variability among signals obtained with different probe sequences, and affect the reproducibility among samples. Short target fragments may work better, because they contain more probe binding sites per nanogram than long targets (see Fig. A).