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What are the settings for yeast lysis with the BigPrep Adapter?

April 26, 2017adapter lysis settings yeast

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What are the settings for yeast lysis with the BigPrep Adapter?

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The FastPrep®-24 instrument in combination with the BigPrep Adapter has been successfully used for the lysis of Pichia Pastoris, a yeast strain that has a big cell wall. After centrifugation of 1 liter culture (5x 10e8 cells/ml), the cell pellet is resuspended in 50 ml Lysis Buffer (containing protease inhibitors and PMSF). 25ml of this solution is added to 2 Big Lysing Matrix B tubes (containing silica beads) and samples are homogenized 4 times for 30 seconds at speed 6.0 m/s. Tubes were incubated on ice for 2 minutes between each run. 80% of yeast cells were lysed.