What are the scientific principles of chromatography?
The easiest way for me to understand this stuff is I remember that like dissolves like. When trying to separate two molecules with different properties (such as one polar and one non-polar molecule) it is possible to do so using chromatography. If you are using a polar stationary phase whichever molecule is more polar will spend more time ‘dissolved’ in the stationary phase which means it will move through the column slower since the more time a molecule spends in the mobile phase the quicker it will move through the column.
