What are the sample requirements for sequencing?
• Genomic DNA requiring nebulization: >5 g of double-stranded DNA (dsDNA) in a 10 L volume. • DNA fragments 200 – 700 bp: >3 g of dsDNA in a 10 L volume. • PCR amplicons that incorporate our fusion primers (<500 bp): ~100 ng of dsDNA in a 10 L volume. 2. What quality of DNA is required? A spectroscopic measurement of the A260/280 ratio of ~1.8 provides a good estimate of sample quality. We also request a 2% agarose gel image of the DNA sample(s) run with a 1 kb ladder to assess incoming sample quality in comparison to our internal QC. 3. Will you check the quality of my DNA before sequencing? All incoming sample quantity is determined by fluorometry and quality is evaluated using either an Agilent bioanalyzer or agarose gel to assess the size distribution of the samples. If sample quantity or quality is not adequate, a replacement sample will be requested before proceeding with the project. 4. What method of quantification should I use? Fluorometry provides the most reproducible and ac
