What are the requirements for conducting rodent survival surgery?
• Is the mouse ascites method an acceptable method of monoclonal antibody production? The NIH concurs with the findings and recommendations in the 1999 report of the National Research Council Monoclonal Antibody Production which indicates that during the accumulation of ascites there is likely to be pain and distress, particularly when some cell lines that are tissue-invasive are used and in situations of significant ascites development. The Report concluded that there is and will continue to be scientific necessity for this method, but that as tissue-culture systems are further developed, tissue-culture methods for the production of monoclonal antibodies should be adopted as the routine method unless there is a clear reason why they cannot be used. Accordingly, IACUCs are expected to critically evaluate the proposed uses of the mouse ascites method. Prior to approval of such protocols IACUCs must determine that (i) the proposed use is scientifically justified, (ii) methods that avoid
In accord with the Guide, the species of animal influences the components and intensity of the surgical program and modification of standard techniques might be desirable or even required, but should not compromise the well-being of the animals. It further notes that some characteristics of common laboratory-rodent surgery, such as smaller incision sites, fewer personnel in the surgical team, manipulation of multiple animals at one sitting, and briefer procedures, can make modifications in standard aseptic techniques necessary or desirable. For most rodent surgery, a facility may be small and simple, such as a dedicated space in a laboratory appropriately managed to minimize contamination from other activities in the room during surgery. (Guide, pages 63 and 78) • Is the mouse ascites method an acceptable method of monoclonal antibody production? The NIH concurs with the findings and recommendations in the 1999 report of the National Research Council Monoclonal Antibody Production whic
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- What standards does the PHS Policy require our institution to follow in conducting survival rodent surgery, and how do they differ from those applicable to other species?
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- What are the requirements for conducting rodent survival surgery?
