What are the required materials to perform Real-Time PCR?
SmartCycler® & software, computer configured for SC, PCR tubes, primers, probe or intercalating dye, dNTP’s, MgCl2, DNA or cDNA, polymerase, water, centrifuge, pipet and pipet tips, buffers. SmartMix and OmniMix are available for convenience which contain all the reagents to perform a PCR reaction, except the primers and probes, in a SmartBead format.