What are the recommended transfection amounts?
We have seen no differences in the transfection efficiency of Silencer Select siRNAs compared to unmodified siRNAs. However, Silencer Select siRNAs are 5–100 fold more potent than other siRNAs, so recommended transfection amounts are about 5–10 fold less than recommended for Silencer siRNAs (depending upon cell type used). For instance, most customers used Silencer siRNAs at ~30 nM in HeLa cells. Silencer Select siRNAs can readily be used at 3–5 nM in HeLa cells without compromising knockdown levels.
