What are the recommended storage conditions of recombinant Adenoviruses?
For long-term storage, the virus should be kept at -80°C, especially after CsCl or chromatography purification. At -80°C, the virus could be stable for 6 months to a year and in some cases even up to 2 years. If the virus is in DMEM supplemented with serum or BSA and stored at -80°C, it will remain stable for longer periods of time and through several freeze-thaw cycles. On the other hand, if the virus CsCl-purified and kept in PBS or Tris solution (20mM Tris PH8.0, 200 NaCl, 2-3% glycerol or sucrose), repeated freeze-and-thaw should be avoided, since it will cause significant decrease of titer. Q. What is the capacity of cloning into the Adenovirus as an expression system? The cloning capacity for the transgene, by using (DE1/E3) adenovirus type 5, is about 8 Kb in length. Q. There are many serotypes of adenoviruses, which one is the most commonly used for gene delivery? The most commonly used adenovirus for gene delivery is human adenovirus serotype 5 (DE1/E3), which is the one that
