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What are the recommended primary antibody incubation times and temperatures?

April 26, 2017Antibody incubation primary recommended temperatures times

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What are the recommended primary antibody incubation times and temperatures?

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A. Generally, BioVision recommends 1 hour incubation with the primary antibody at room temperature. This is the optimized condition at BioVision, however overnight incubation of the antibody at 4°C could also work well in some cases.