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What are the recommended carbon dioxide (CO2) levels needed to grow a cell culture?

April 26, 2017carbon dioxide CELL CO2 culture grow interview question interview questions levels needed recommended

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What are the recommended carbon dioxide (CO2) levels needed to grow a cell culture?

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While the levels of carbon dioxide in cell culture systems vary from that in ambient air (about 0.03%) up to 40% in air, generally either no added CO2 or 5% to 10% CO2 in air are the most frequently used.