What are the pSpark DNA cloning systems?
A1. The pSpark DNA cloning systems are a family of cloning vectors for blunt end DNA cloning. They are based on a novel patent pending technology for background reduction. Vectors are digested with EcoRV and both ends are treated to reduce background to less than 1% . They are not T-vectors. Also, they are not based on transcription of toxic genes to reduce background and for this reason, ORF and promoters can also be cloned into pSpark DNA cloning systems without cloning bias or artifacts.
