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What are the probes used to design the NCode multi-species miRNA microarray V2?

April 26, 2017design microarray mirna multi-species probes Used

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What are the probes used to design the NCode multi-species miRNA microarray V2?

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The NCode™ Multi-Species miRNA Microarray V2 consists of oligonucleotides complementary to known miRNA sequences for human, mouse, rat, D. melanogaster, C. elegans, and Zebrafish. These species-specific, unmodified oligonucleotides are designed from the miRNA sequences in the Sanger miRBase Sequence Database, Release 9.0. The array also includes probes for some small nucleolar RNAs. Each miRNA probe is synthesized as a tandem repeat and is spotted in triplicate. The microarray is also spotted with control probes for a unique miRNA sequence that has been designed not to cross react with endogenous miRNAs. In addition, there is a dye control printed in a range of concentrations from high to low, to allow for dye normalization durning scanning. The NCode™ Human miRNA Microarray V3 contains optimized probes to the known mature miRNAs in the miRBase Sequence Database, RElease 10.0 for human. In addition, there are probes to 373 novel human miRNAs discovered through deep sequencing, and biol