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What are the prerequisites to use quantification by UV?

April 26, 2017prerequisites Quantification UV

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What are the prerequisites to use quantification by UV?

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Quantification by UV is only possible for compounds with significant absorbance above 250 nm due to the background absorbance of most commercially available translucent plates and DMSO. We recommend centrifugation of UV plates for 2 minutes at 750 g to destroy any air bubbles that otherwise may cause light scattering.