What are the precipitates that settled on top of the cells after adding the DNA/GenePORTER Lipoplexes, which are visible under light microscopy?
After adding the DNA/GenePORTER complex to the cells, there may be grainy sand-like precipitates settled on the tops of the cells. This is a common observation among all lipid-based transfection reagents. “Precipitates” on top of the cells are the actual DNA/GenePORTER complexes attached to the cell surface via electrostatic interaction. The uptake of the desired DNA could not be achieved without the complexes coming into direct contact with cell surface.
