Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

What are the possible effects of air bubbles in the final 2D map?

April 26, 2017air bubbles final map possible

0

Posted

What are the possible effects of air bubbles in the final 2D map?

1 Answer

0

Posted

A. Air bubbles trapped between the IPG Strip and the second-dimension gel surface may cause vertical gaps in the 2-D map as well as irregular dye fronts. Ensure that no bubbles are trapped between the IPG strip and the top surface of the second-dimension gel. If using a flatbed gel format (i.e. Multiphor II), stroke the plastic backing of the IPG strip gently with a pair of forceps to remove any bubbles. Find more information on our Immobiline DryStrips here.