Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.
TMB is a faster developing, stronger substrate (yielding greater amplification/sensitivity/background); use of stop solution produces a yellow end product read at 450 nm. ABTS is a slower-developing blue/green substrate (read at 405 nm), particularly useful for kinetic studies (multiple readings).
