What are the pDNR-Dual, pDONR201, pDONR221, etc. vectors?
A. Most clones generated in the LaBaer laboratory and shared with the repository are based on successful recombinational cloning methodologies such as the Clontech Creator system (LoxP sites) or the Invitrogen Gateway system (att sites). These flexible systems allow a single sequence-verified ORF (“master” or “donor” clone) to be used to create expression constructs for a variety of different techniques via recombination (few steps) rather than restriction enzyme cloning and ligation (several steps). In general, we keep the master clones in our collection because they give the most flexibility to the largest set of users. But in some cases, we also have expression versions. “Entry” or “acceptor” vectors used in combination with master clones to create expression constructs are available from a number of different sources, including Clontech and Invitrogen.
