What are the optimal reaction conditions for my protein?
The binding conditions for each experiment must be optimized empirically for the proteins tested. Journal articles are an excellent source for information on how to set up EMSA binding reactions. If an article describes an EMSA for your protein of interest but used 32P labeled probes, repeat the binding conditions with biotinylated probe in place of the 32P labeled probe. Although reaction components are provided with the LightShift Kit, these reagents may not be suitable for individual applications and may be adjusted as needed.
