What are the OD600 values at the normal shaking speed?
The normally-used shaking speed for E.coli growth is 250 rpm in most labs. At 250 rpm, the high density growth media can only reach about OD600 = 10. The OD values will be lower at speeds slower than 250 rpm. At low shaking speeds (<350 rpm), the oxygen concentration is limited in the medium and the cells will produce large quantity of acids which will make the medium acidic. Therefore, it is not recommended to use high density growth media at low shaking speeds.
