What are the mass ranges of substrates/products than can be detected using RapidFire®?
We routinely assay over a wide range of masses including small molecule peptides up to about 40 amino acids in length. The mass range is determined by the mass spectrometer that is used for analyte quantitation. Most commercially available triple quadrupole mass spectrometers have an upper limit of about 3000 amu. Since these instruments can measure multiply charged species, we can look at larger molecules. Using RapidFire with a Time-of-Flight (TOF) instrument enables us to detect proteins of 150kb and larger. Please contact us to discuss your assay needs as we have extensive experience quantitating various molecules.
