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What are the major differences between the miRNA and DASL protocols?

April 26, 2017dasl differences mirna protocols

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What are the major differences between the miRNA and DASL protocols?

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There are four new reagents, Polyadenylation Single (PAS), cDNA Synthesis Single (CSS), miRNA Assay Pool (MAP) and Single-Color Master Mix (SCM). There are also two new steps in the protocol: “Make PAP” for the polyadenylation and “Make CSP” for cDNA synthesis. In addition, the “Make ASE” and “Cycle PCR” steps are modified.