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What are the main differences between HILIC columns and a Cogent TYPE-C silica based column?

April 26, 2017based cogent column columns differences main silica type-c

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What are the main differences between HILIC columns and a Cogent TYPE-C silica based column?

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[top] There are many differences between HILIC columns and Cogent TYPE-C columns but for the sake of brevity, I will only answer with the main differences. The Cogent TYPE-C columns all perform similarly to HILIC as far as polar compound elution order is concerned when using higher than 70% organic composition of the mobile phase. Both columns perform separations that are based on variations of Normal phase called Aqueous Normal Phase. The HILIC stationary phase is typically more polar then the TYPE-C bonded phase which is relatively non polar. HILIC columns will retain polar compounds that cannot be retained by other columns and so will the TYPE-C columns. The HILIC columns will not retain non-polar compounds. This is one of the big advantages for the TYPE-C columns, where you will be able to separate polar and non-polar compounds in the same isocratic run. On HILIC columns, polar compounds partition into and out of the hydration shell created by adsorbed water on the silica surface.