What are the limitations of deconvolution software like MicroTome?
The principal limitation of the digital deconvolution approach has been the amount of computer time required to deconvolve a single slice. Until now, a single slice could require several minutes to deconvolve on a personal computer. A large data set could take an entire day to process. With the introduction of MicroTome, however, processing time has been reduced to no more than a few seconds per slice on a PC or Power Mac. These speeds are possible because of VayTek’s unique, efficient implementations of the algorithms. There is an additional limitation with MicroTome. Slices must be relatively close to each other to achieve the proper resolution after deconvolution. The exact distance will vary from sample to sample, but experience has shown that it should be between .1 micron and 10 microns.
