What are the likely causes of poor quality sequence?
The most common causes of poor quality sequence are: specific and unique sequences poor quality template too much DNA too little DNA It is not uncommon for the sequence to die after long runs of poly A/T sequence due to the polymerase falling off the template DNA. Contact PAN for assistance. High G/C content does not appear to be a major problem for the newer cycle sequencing kits. Occasionally, template secondary structure can cause sequencing problems.
