Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

What are the key differences between the protocols for genotyping using the GoldenGate Assay and the GoldenGate Assay for Methylation?

April 26, 2017assay differences Genotyping goldengate key Methylation protocols

0

Posted

What are the key differences between the protocols for genotyping using the GoldenGate Assay and the GoldenGate Assay for Methylation?

1 Answer

0

Posted

DNA samples require bisulfite conversion prior to use in the GoldenGate Assay for Methylation but not for genotyping using the GoldenGate Assay. There are four oligos, two allele-specific oligos (ASOs), and two locus-specific oligos (LSOs) required for each assay locus in the Oligo Pool for Methylation Assay (OMA): one probe pair for the methylated state of the CpG site and a corresponding pair for the unmethylated state. The GoldenGate Assay for Methylation is not LIMS-supported. Analysis of methylation data requires the BeadStudio Methylation module.