Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

What are the incubation temperatures for each step?

incubation step temperatures
0
10 Posted

What are the incubation temperatures for each step?

0
10

First strand primer annealing = 65 °C First strand synthesis = 25 °C and 42 °C Second strand synthesis = 25 °C and 50 °C Post second strand Enhancement = 37 °C SPIA™ amplification = 47 °C

Related Questions

What is your question?

*Sadly, we had to bring back ads too. Hopefully more targeted.

Experts123