What are the image statistics? How are they calculated?
The image statistics optionally display the minimum intensity, maximum intensity, mean intensity and a histogram depicting the distribution of intensities in a microscopy image. The values are determined by reading the intensity value for every pixel in the image. The histogram depicts the frequencies of all possible intensity values in the image. The scale of the x-axis is 0 to 65535 (the possible intensity values in a 16-bit greyscale image). The scale of the y-axis is the ratio of the number of pixels with a given intensity to the number of pixels for the intensity with the most pixels. The y-axis essentially depicts the frequency of a given intensity relative to the most frequent intensity. NOTE: If scaling is turned ON, the statistics and histogram are derived from the scaled image.