What are the fluorescent proteins are used for the organelle markers?
The fluorescent proteins are turboGFP and turboRFP from Evrogen. Both proteins are super bright with brightness % compared to EGFP of 112 and 187, respectively. They are both fast maturing, allowing detection of fluorescence as early as 8-10 hrs post transfection. In spite of their dimeric structures, tGFP and tRFP have been successfully used as fusion protein for subcellular structure labeling.
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