What are the fluorescent lifetimes of the Alexa Fluor dyes?
• The fluorescence lifetime (τ) of the Alexa Fluor 647 dye in H2O at 20°C is 1.0 nanoseconds and 1.5 nanoseconds in EtOH. • The fluorescence lifetime (τ) of the Alexa Fluor 680 dye in pH 7.5 buffer at 20°C is 1.2 nanoseconds. • The fluorescence lifetime (τ) of the Alexa Fluor 750 dye in H2O at 22°C is 0.7 nanoseconds.
